Already established as a classic comprehensive reference for the whole team at the IVF clinic, this new edition has been extensively revised, with the addition of several important new contributions on laboratory ( including advanced sperm selection techniques for ICSI, human embryo biopsy procedures, oocyte activation, managing an oocyte bank, artificial gametes, and epigenetics) as well as on clinical topics (including GnRH agonist triggering, segmentation of IVF treatment, uterus transplantation, and risk and safety management). As previously, methods, protocols, and techniques of choice are presented by eminent contributors internationally.

Features

• Confirms its position as the leading reference in the field
• Contributed by a range of international experts and leading researchers
• Is illustrated in colour throughout
• Contains video illustrations of new techniques in the integral ebook
• Is without equal as a comprehensive one-stop reference

About the Author

Contents

۱. New guidelines for setting up an assisted reproduction technology laboratory
۲. Quality control: Maintaining stability in the laboratory
۳. The assisted reproduction technology laboratory: Current standards
۴. Evaluation of sperm
۵. Sperm preparation techniques
۶. Sperm chromatin assessment
۷. Oocyte retrieval and selection
۸. Preparation and evaluation of oocytes for intracytoplasmic sperm injection
۹. Advanced sperm selection techniques for intracytoplasmic sperm injection
۱۰. Use of in vitro maturation in a clinical setting: Patient populations and outcomes
۱۱. Intracytoplasmic sperm injection: Technical aspects
۱۲. Assisted hatching
۱۳. Human embryo biopsy procedures
۱۴. Assisted oocyte activation: Current understandingr practice, and future perspectives
۱۵. Analysis of fertilization
۱۶. Culture systems for the human embryo
۱۷. Evaluation of embryo quality: Analysis of morphology and physiology
۱۸. Evaluation of embryo quality: Time-lapse imaging to assess embryo morphokinesis
۱۹. Evaluation of embryo quality: Proteomic strategies
۲۰. The human oocyte: Controlled-rate cooling
۲۱. The human oocyte: Vitrification
۲۲. The human embryo: Slow freezing
۲۳. The human embryo: Vitrification
۲۴. Managing an oocyte bank
۲۵. Severe male factor infertility: Genetic consequences and recommendations for genetic testing
۲۶. Polar body biopsy and its clinical application
۲۷. Preimplantation genetic diagnosis for infertility
۲۸. Genetic analysis of the embryo
۲۹. Diagnosis of endometrial receptivity and the embryo-endometrial dialog
۳۰. Artificial gametes: The oocyte
۳۱. Microfluidics in assisted reproduction technology: To’wards automation of the in vitro fertilization laboratory
۳۲. Epigenetic considerations in preimplantation mammalian embryos
۳۳. Quality management in reproductive medicine
۳۴. Lifestyle r periconception, and fertility
۳۵. The environment and reproduction
۳۶. Indications for in vitro fertilization treatment: From diagnosis to prognosis
۳۷. Initial investigation of the infertile couple
۳۸. Prognostic testing for ovarian reserve
۳۹. Drugs used for ovarian stimulation: Clomiphene citrate,, aromatase inhibitors,, metformin, gonadotropins, gonadotropin-releasing hormone analogs, and recombinant gonadotropins
۴۰. The role of follicle-stimulating hormone and luteinizing hormone in ovarian stimulation: Current concepts
۴۱. Endocrine characteristics of assisted reproduction technology cycles
۴۲. The use of gonadotropin-releasing hormone agonists and the efficiency of in vitro fertilization
۴۳. Gonadotropin-releasing hormone antagonists in ovarian stimulation for in vitro fertilization
۴۴. Gonadotropin-releasing hormone agonist triggering
۴۵. Segmentation of in vitro fertilization treatment
۴۶. The use of ovarian reserve biomarkers to tailor ovarian stimulation for in vitro fertilization
۴۷. Monitoring ovarian response in assisted reproduction (in vitro fertilization and intracytoplasmic sperm injection)
۴۸. Oocyte collection
۴۹. Luteal-phase support in assisted reproduction technology
۵۰. Treatment strategies in assisted reproduction for the poor-responder patient
۵۱. Recurrent implantation failure
۵۲. Ultrasonography in assisted reproduction
۵۳. Sperm recovery techniques: Clinical aspects
۵۴. Processing and cryopresen/ation of testicular sperm
۵۵. Embryo transfer technique
۵۶. Cycle regimes for frozen-thawed embryo transfer
۵۷. Anesthesia for in vitro fertilization
۵۸. Medical considerations of single-embryo transfer
۵۹. Endometriosis and assisted reproduction technology
۶۰. Polycystic ovary syndrome and assisted reproduction
۶۱. Management of hydrosalpinx
۶۲. Fertility presen/ation strategies
۶۳. Uterus transplantation
۶۴. Viral disease and assisted reproduction technology
۶۵. Severe ovarian hyperstimulation syndrome
۶۶. Bleeding, severe pelvic infection, and ectopic pregnancy
۶۷. Iatrogenic multiple pregnancies: The risk of assisted reproduction technology
۶۸. Egg and embryo donation
۶۹. Gestational surrogacy
۷۰. The evolving role of the assisted reproduction technology nurse: A contemporary review
۷۱. Patient support in the assisted reproduction technology program
۷۲. The relationship between stress and in vitro fertilization outcome
۷۳. The impact of legislation and socioeconomic factors in the access to and global practice of assisted reproductive techniques
۷۴. Religious perspectives on human reproduction
۷۵. Risk and safety management in assisted reproduction technology